PFKM anticorps

N° du produit ABIN458975

L’anticorps Mouton Polyclonal anti-PFKM a été validé pour WB, ELISA et IF. Il convient pour détecter PFKM dans des échantillons de Lapin.

Aperçu rapide pour PFKM anticorps (ABIN458975)

Antigène: PFKM (phosphofructokinase, Muscle (PFKM))

Reactivité: Lapin

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp PFKM est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Détail du produit anti-PFKM anticorps

Specificité: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Attributs du produit: Affinity purified antibodies of polyclonal sheep antiserum to fructose-6-phosphate kinase from rabbit muscle

Purification: Specific polyclonal antibody fractions were prepared by solid phase affinity chromatography.

Immunogène: Fructose-6-phosphate kinase isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Information d'application

Indications d'application: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: PAb concentration 1 mg/0.5ml. No foreign proteins added.

Buffer: Purified hyperimmune sheep IgG antibodies lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2), stabilized with dextran.

Agent conservateur: Without preservative

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilised PAb fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 0.5 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Détails sur PFKM

Antigène: PFKM (phosphofructokinase, Muscle (PFKM))

Autre désignation: Fructose-6-phosphate Kinase

Sujet: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.

Pathways: Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, L'effet Warburg